Example of poorly trimmed sequences (sequence four and on) from the species Setosphaeria turcica. The 5’ end of the alignment is shown, and the poorly trimmed sequences cover the last ~5 bases of SSU and the immediate start of ITS1. The topmost sequence is genome-derived, and sequences two and three are regular Sanger sequences retrieved from the INSDC from other studies than the one with the poorly trimmed sequences (sequences four and on). SeaView v. 4 (Gouy et al. 2010) was used to visualize the alignment.