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  • ContentsContents
  • Article InfoArticle Info
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  • Article title
  • Abstract
  • Key words
  • Introduction
  • Materials and methods
    • Sample collection and pathogen isolation
    • Morphological analysis
    • DNA extraction, PCR amplification, and sequencing
    • Sequence alignment and phylogenetic analysis
    • Library preparation, genome sequencing, and assembly
    • Gene prediction and functional annotation
    • Phylogenetic tree reconstruction, genome alignment, divergence time estimation, and gene family analysis
  • Results and discussion
    • Phylogenetic analyses
    • Taxonomy
    • Synteny analysis and genome assembly characteristics
    • CWDE and BSGC contents reveal lifestyles and pathogenicity potential of Cytospora piceae EI-19(A) and C. piceicola EI-20
    • Cytospora piceae EI-19(A) and C. piceicola EI-20 experienced gene family contraction during genome evolution
    • Cytospora piceae EI-19(A) and C. piceicola EI-20 constrain specific secretomes and effectomes within Diaporthales
    • Cytospora piceae EI-19(A) and C. piceicola EI-20 harbor a wide range of virulence-related genes
  • Conclusion
  • Acknowledgments
  • Additional information
    • Conflict of interest
    • Ethical statement
    • Funding
    • Author contributions
    • Author ORCIDs
    • Data availability
  • References
  • Supplementary material
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