Corresponding author: Wen-Ying Zhuang (
Academic editor: A. Miller
A new species,
Zheng H-D, Zhuang W-Y (2017)
In China, only two Vibrisseaceous fungi were recorded, i.e.
Specimens were collected from Beijing and Yunnan Province in 2016, and apothecial gross morphology when fresh was photographed by a Canon PowerShot G16 digital camera. Dried apothecia were rehydrated with distilled water and sectioned at a thickness of 15 μm with a Yidi YD-1508A freezing microtome (Jinhua, China). Measurements were taken from longitudinal sections and from squash mounts in lactophenol cotton blue solution using an Olympus BH-2 microscope (Tokyo, Japan). Iodine reactions of ascus apparatus were tested in Melzer’s reagent and Lugol’s solution with or without 3% KOH solution pretreatment according to
Genomic DNA was extracted from dried apothecia using Plant Genomic DNA Kit (TIANGEN Biotech. Co., Ltd., Beijing, China). Materials were crushed in liquid nitrogen before extraction. The internal transcribed spacer of nuclear ribosomal DNA (
Sequences used in this study.
Species | Strain/specimen |
|
---|---|---|
CBS 109314 |
|
|
BB11_301_Ah_Pi_150506 (DNA46) |
|
|
VL272 |
|
|
CBS:309.71 |
|
|
PDD 75692 |
|
|
|
PDD:88457 |
|
ICMP19895 |
|
|
PDD 99891 |
|
|
PDD:83226 |
|
|
PDD70070 |
|
|
M235 |
|
|
M39 |
|
|
ARON3113.P |
|
|
3706 |
|
|
DAOM C 250106 |
|
|
DAOM C 250108 |
|
|
CBS 300.62 |
|
|
JLF2084 |
|
|
MBH39316 |
|
|
|
N/A |
|
|
|
|
PDD 99892 |
|
|
PDD 99893 |
|
|
Uncultured |
WPD-OTU-39 |
|
Uncultured |
WPW-OTU-33 |
|
PDD 81292 |
|
|
|
||
BM18#13 |
|
|
|
||
B 70 0009352 |
|
|
MBH29259 |
|
|
TNS:F-31802 |
|
|
WZ0024 |
|
|
ARON2329.P |
|
* Numbers in bold indicate sequences produced by this study
The
Phylogenetic affinity of
CHINA, Yunnan Province, Maguan County,
The specific epithet is in memory of the late distinguished mycologist Dr. R.P. Korf.
Apothecia scattered or in cluster, discoid to slightly convex, stipitate, light greenish yellow, with hymenium and receptacle surface concolorous when fresh, 0.8–2.0 mm in diam. and 0.6–1.5 mm high, dried apothecial tissues with exudation of light yellow pigment in water; stipe cylindrical, with base dark, 0.4–1.0 mm high. Ectal excipulum of textura prismatica, non-gelatinous, 30–100 µm thick, hyphae parallel or lying at a low angle to receptacle surface, cells hyaline, with walls slightly thickened, terminal cells of external hyphae thin-walled, 8–25 × 3–7 μm. Medullary excipulum of textura porrecta to textura intricata, 30–300 µm thick, hyphae hyaline, 3–5 µm wide. Subhymenium not distinguishable. Hymenium 95–110 µm thick. Asci arising from simple septa, 8-spored, cylindrical, J+ in Melzer’s reagent and Lugol’s solution without KOH pretreatment, visible as two short blue lines, 70–83 × 4.5–5.5 μm. Ascospores filiform, tapering slightly from rounded apex to pointed base, hyaline, smooth, multiguttulate, non-septate, in fascicle, 44–52 × 1.2–1.5 μm. Paraphyses filiform, hyaline, unbranched, 1.5–2.0 µm wide, not exceeding the asci.
Apothecia scattered or in cluster, slightly convex, sessile, hymenium surface light yellow, 0.5–1.5 mm in diam., receptacle surface brownish. Ectal excipulum of textura angularis to textura prismatica, non-gelatinous, lying at a high angle to the outer surface, 30–140 µm thick, inner cells subhyaline to light brown and outer cells brown, 15–30 × 9–14 μm. Medullary excipulum of textura angularis to textura prismatica, 50–150 µm thick, cells hyaline, 5–16 × 4–6 μm. Subhymenium not distinguishable. Hymenium 280–290 µm thick. Asci arising from simple septa, 8-spored, cylindrical, J– in Melzer’s reagent and Lugol’s solution with or without KOH pretreatment, 227–241 × 5–6 μm. Ascospores filiform, hyaline, smooth, multiguttulate, multi-septate, break into several pieces, in fascicle, 192–208 × 1.5–2.0 μm. Paraphyses filiform, slightly enlarged at the apex, hyaline, branched at upper portion, 3.0–5.0 µm wide at apex and 2.0–2.5 μm wide blow, exceeding the asci by 20–35 μm.
CHINA, Beijing, Yunmeng Moutain,
This is the first report of
The three sexual genera in
In the family
The two asexual genera are recognizable and associated each other with low supports (Fig.
In conclusion,
This work was supported by the National Natural Science Foundation of China (nos. 31570018, 31300021). The authors would like to thank Dr. X.H Wang and Dr. S.H. Li for their invaluable help during the field work.