The phylogenetic tree of Simplicillium and Leptobacillium was inferred from six-gene dataset (ITS, nrSSU, nrLSU, tef-1α, rpb1, rpb2), based on the Bayesian inference and the maximum likelihood analyses. Each value at a node indicates a bootstrap proportion (the left) and Bayesian posterior probability (the right). The scale bar 0.06 indicates the number of expected mutations per site. The species in bold black font of the Simplicillium and Leptobacillium were from this study. B. bassiana ARSEF 1564 and B. brongniartii ARSEF 617 were designated as outgroups.