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A Cartoon representation of the maximum likelihood ITS topology obtained from 240 Rhizoplaca melanophthalma sensu lato specimens in Leavitt et al. (in review). Values at each node indicate non-parametric-bootstrap support; only support values > 50% are indicated B Box plots of ITS genetic distances within each new species, all intraspecific distances, and all interspecific distances. In each box plot, the box shows the interquartile range (IQR) of the data. The IQR is defined as the difference between the 75th percentile and the 25th percentile. The solid and dotted line through the box represent the median and the average length, respectively; and C The coalescent-based species-tree for the Rhizoplaca melanophthalma species-complex estimated from five genetic markers (ITS, IGS, group I intron, β-tubulin, and MCM7 loci) in Leavitt et al. 2011a.