Phylogram generated from Maximum Likelihood analysis, based on combined LSU, SSU and tef1-α sequence data of 41 taxa, which comprised 2836 base pairs (LSU = 902 bp, SSU = 1031 bp, tef1-α = 903 bp). The best scoring RAxML tree with a final likelihood value of -14798.632437 is presented. The matrix had 1013 distinct alignment patterns, with 24.90% of undetermined characters or gaps. Estimated base frequencies were as follows: A = 0.241740, C = 0.258134, G = 0.292403, T = 0.207722; substitution rates: AC = 0.834723, AG = 2.021967, AT = 1.126143, CG = 1.032150, CT = 7.231944, GT = 1.000000; gamma distribution shape parameter α = 0.320795. Bootstrap support values for ML equal to or greater than 60% and Bayesian Inference analysis values equal to or greater than 0.90 PP are labelled at each node. The tree is rooted with Anisomeridium phaeospermum (MPN539) and A. ubianum (MPN94). Related sequences were collected following Li et al. (2022), Kularathnage et al. (2023) and Dong et al. (2023). The new isolates are indicated in red and the ex-type strains are in bold. Group A indicates the taxa used to compare the morphology with our new species (Melomastia puerensis).