Corresponding author: Chu-Long Zhang (clzhang@zju.edu.cn)
Academic editor: N. Boonyuen
Zhao R, Mao L-J, Zhang C-L (2023) Three new species of
The genus
Traditionally,
Fungal diversity is enormous in China (
In accordance with a prior study by
All strains of
The morphological observation of the colonies was based on strains grown on potato dextrose agar (
Microscopic preparations were made by mounted on lactic acid, and at least 30 measurements per structure were documented and examined under a Nikon Eclipse 80i microscope (Nikon Corp.). Length (L) and width (W) of the phialides, conidia and chlamydospores were measured, respectively, and the ratio of length to width was calculated. Measurement values are expressed as (a–)b–c(–d), where (a) represents the lowest extreme value, b–c contains the minimum value of 90% of the calculated values, and (d) denotes the highest extreme value. The letter “n” indicates the total number of measurements taken (
The mycelia of pure cultures were scraped directly from plates after 2–3 d growth on
Strain numbers and corresponding GenBank accession numbers of sequences used for phylogenetic analyses.
Species name | Strain number | GenBank accession numbers | |
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CBS 124620 ET |
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GJS 04–193 |
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YMF 1.00383 T |
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YMF 1.00168 |
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YMF 1.00352 T |
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CBS 548.92 T | – |
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GIS 04–67 |
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GJS 05–101 |
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CBS 119499 |
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CBS 142.95 ET |
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HMAS 248845 |
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CBS 131803 T |
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HGUP 0039 |
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CBS 226.95 ET |
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TRS55 |
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TRS94 |
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YMF 1.06211 T |
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YMF 1.6190 |
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CBS 136489 T |
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S489 |
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YMF 1.04628 T |
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JZBQH12 T |
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JZBQH11 |
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CBS 135574 ET |
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T20 |
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YMF 1.06201 T |
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YMF1.6180 |
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YMF 1.04622 T |
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TRS575 |
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CBS 119325 ET |
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YMF 1.00268 ET |
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CBS 121551 T |
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CBS 299.78 ET |
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Note: Newly–sequenced material is indicated in bold type. T Indicates a type culture. ET Indicates an epitype culture.
The phylogeny was constructed with the concatenated sequences of
The comparison of
The similarity of
Query species | Related species | Sequences similarity value(%) | |
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97.91 | 91.29 | ||
98.15 | 86.68 | ||
98.65 | 87.53 | ||
98.56 | 94.40 | ||
97.54 | 99.20 | ||
96.92 | 98.06 | ||
97.05 | 98.29 | ||
97.42 | 99.16 | ||
97.17 | 97.83 | ||
97.54 | 99.20 | ||
97.79 | 98.06 | ||
97.17 | 98.29 | ||
98.04 | 100 | ||
97.66 | 97.83 |
Note: T Indicates a type culture. ET Indicates an epitype culture.
Multi-loci phylogenetic analyses were performed on sequences obtained from 43 strains, consisting of 30 strains from the
Phylogenic tree generated by the maximum likelihood analysis using the concatenated sequences of
The Latin specific epithet “
Phylogenetically,
Cultures and anamorph of
China: Shandong Province, Dezhou City,
Optimal growth at 25 °C, slow at 35 °C on all media.
Colony radius on
Colony radius on
Colony radius on
Colony radius on
Unknown.
Soil.
China, Shandong Provinces.
China: Shandong Province, Jinan City,
The Latin specific epithet “
It is easily distinguished from these related species by its relatively large chlamydospores (11.7–)13.3–16.4 (–19.5) × (11.5–)12.8–14.6–12.8 (–16.0) μm (mean = 14.8 × 13.6 μm) (n = 30).
Cultures and anamorph of
China: Shandong Province, Weifang City,
Optimum temperature for growth is 30 °C on
Colony radius on
Colony radius on
Colony radius on
Colony radius on
Unknown.
Soil.
China, Shandong and Shanxi provinces.
China: Shandong Province, Jinan City,
Although
The Latin specific epithet “
Cultures and anamorph of
China: Shanxi Province, Jincheng City,
Optimum temperature for growth is 30 °C on
Colony radius on
Colony radius on
Colony radius on
Colony radius on
Unknown.
Soil.
China, Shanxi Province.
China: Shanxi Province, Jincheng City,
Similar species can be distinguished according to the pigment:
All three new species were isolated from soils. Based on morphology and phylogenetic analyses, the taxonomic positions of three new species were explored. Of these species,
The genus
Numerous biological control agents have been derived from species in the
This work was supported by the Provincial Key Research and Development Plan of Zhejiang, China (2020C02027).