PCR amplification and sequencing results of the mtSSU locus in the genus Melanelia A 2% agarose gel electrophoresis of PCR products, where lanes 1-3 contain amplicons from the universal primer pair mrSSU1 and mrSSU3R, and lanes 4-6 are from the mycobiont-specific primer pair mt-SSU-598-5’and mt-SSU-1324-3’. Abbreviations: M-molecular ladder 100 bp; NC-negative control B illustration of sequencing results (M. agnata, voucher number LA29683) using the forward primers: the universal primer mrSSU1 (upper) and the mycobiont-specific primer mt-SSU-581-5’ (lower), respectively.

 
 
  Part of: Xu M, Liu Y, Möller E, LaGreca S, Moya P, Wang X, Timdal E, de Boer H, Barreno E, Wang L, Thüs H, Andrésson Ó, Magnússon KP, Ólafsdóttir ES, Heiðmarsson S (2023) Mycobiont-specific primers facilitate the amplification of mitochondrial small subunit ribosomal DNA: a focus on the lichenized fungal genus Melanelia (Ascomycota, Parmeliaceae) in Iceland. MycoKeys 96: 57-75. https://doi.org/10.3897/mycokeys.96.100037