Mycobiont-specific primers facilitate the amplification of mitochondrial small subunit ribosomal DNA: a focus on the lichenized fungal genus Melanelia (Ascomycota, Parmeliaceae) in Iceland


	Comparison of primer specificity between the new and the universal primer pairs using in silico PCR. The number of off-target amplifications is classified as others in grey, while desired amplifications of lichen-forming fungi in Parmeliaceae are marked in white. The number of nucleotide mismatches in the priming sites is shown as Mis_0 to Mis_3, indicating 0 to 3 nucleotide mismatches.

Part of: Xu M, Liu Y, Möller E, LaGreca S, Moya P, Wang X, Timdal E, de Boer H, Barreno E, Wang L, Thüs H, Andrésson Ó, Magnússon KP, Ólafsdóttir ES, Heiðmarsson S (2023) Mycobiont-specific primers facilitate the amplification of mitochondrial small subunit ribosomal DNA: a focus on the lichenized fungal genus Melanelia (Ascomycota, Parmeliaceae) in Iceland. MycoKeys 96: 57-75. https://doi.org/10.3897/mycokeys.96.100037