Corresponding author: Karen W. Hughes (
Academic editor: S. Redhead
Two species of
Petersen RH, Hughes KW, Justice J (2014) Two new species of
The annual national foray of the North American Mycological Association was held in northern Arkansas in 2013, specifically in the lower Boston Mountains of the Ozark Ecoregion. Among collections of fleshy fungi were several species of
American literature dealing with the modern genus
In two seminal publications,
Microscopic observations employed an Olympus BX60 microscope fitted for bright field phase contrast = Tennessee Fieldbook number, assigned in the field to track notes and photos = Herbarium, University of Tennessee, permanent accession number = spore length divided by spore width = mean Q value of number of spores measured = mean length of all spores measured
The term “firm-walled” is intended to describe hyphal walls which are thick enough so that hyphal crumpling or collapse is not observed in microscopic mounts, but there is no measurable distance between inner and outer wall surfaces. “Thick-walled,” conversely, describes a hyphal wall thick enough that the inner and outer surface are sufficiently distinct and that the wall thickness can be measured and reported.
Procedures for DNA extraction, PCR and DNA sequencing of the ribosomal ITS and LSU regions was performed as described in
United States, Arkansas, Searcy Co., grounds of Shepherd of the Ozarks,
admiratia = surprise, astonish, referring to the acanthodendroid hyphae in the outer stipe context.
1) Member of
Basidiomata of
Basidiomata of
Generally second-growth hardwood forest of
Surface of lower stipe covered with felty white tomentum;
Microstructures of
Superficially, basidiomata of
As noted by
The terms “monomitic,” “dimitic”, etc. were coined by
United States, Arkansas, Baxter Co., vic. Big Flat, Rte 341, Moccasin Creek Trailhead, Ozark National Forest,
Calvus = bald; distalis = referring to the spore wall opposite the hilar appendage.
1) Member of
Adult
Basidiomata of
Possibly associated with deciduous trees from local forests of
Basidia and basidiospores of
Care must be taken to ascertain the condition of basidial septa. In clamped basidia, subsequent basidioles arise through the subtending clamp. In clampless basidia (as above), subsequent basidioles arise in precisely the same fashion, arising just below the subtending septum, but without the telltale evidence of a clamp connection. Additional care is required to ascertain that both tramal hyphae and basidia are without clamps. Spores are among the longest in the subgenus and largely as a result, Qm value is high. Spore outline is distinctly boletoid with slight suprahilar depression. First impression was of delicately marbled stipe flesh (i.e. with small, scattered areas of hyphae with gelatinized walls), but closer examination revealed scattered degeneration of inner stipe flesh through maggot infestation. Lower and middle branches as well as apices are essentially unicolorous, straw yellow or dull ochraceous yellow. Juvenile branches exhibit a slight blush of pale pinkish buff, but this soon fades and slowly turns to the adult yellow shades.
In the three days of the NAMA foray, numerous collections of this species were made. Basidiomata seem to occur in troops and “fairy rings” under deciduous trees and are often somewhat bulky. Stipes are not deeply rooted and are easily dislodged, but adult stipes seem consistently maggot-ridden. Because a new taxon was not anticipated, only the type collection was preserved. Although the literature dealing with
If the key to clampless taxa in
PhyML Phylogeny of proposed new species of
Sincere thanks are extended to Carl Davis and Therese Martin of Louisiana who collected