11urn:lsid:arphahub.com:pub:C004A564-9D6A-5F9F-B058-6A3815DFE9C3MycoKeysMC1314-40571314-4049Pensoft Publishers10.3897/mycokeys.4.28091188Research ArticleAscomycotaAscomycote-containing lichensLichenized FungiPhylogenyTaxonomyIndian Ocean IslandsReunionA further new species in the lichen genus Arctomia: A. borbonica from Reunion (Mascarene archipelago)
MagainNicolas1nicolas.magain@ulg.ac.beSérusiauxEmmanuël1Evolution and Conservation Biology Unit, University of Liège, Sart Tilman B22, B-4000 Liège, BelgiumUniversity of LiegeLiegeBelgium
Corresponding author: Nicolas Magain (nicolas.magain@doct.ulg.ac.be)
Academic editor: Imke Schmitt
201220720124921FFCCFF81-FFDE-AA67-FFA2-FFE7FFD5FF9F5751791220121862012Nicolas Magain, Emmanuël SérusiauxThis is an open access article distributed under the terms of the Creative Commons Attribution License 3.0 (CC-BY), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Arctomia borbonicasp. nov. is described as new for science from montane natural and secondary habitats in Reunion in the Mascarene archipelago (Indian Ocean). It has a sterile, foliose, usually wrinkled, thallus whose margins produce goniocysts that disintegrate into a soredioid margin; it looks like a Leptogium species. Its phylogenetic position in the Arctomiaceae (Ostropomycetidae, Ascomycota) has been determined with 3 genes (nuLSU, mtSSU, RPB1) inferences.
Within the Lecanoromycetes, the subclass Ostropomycetidae Reeb, Lutzoni and Cl. Roux exhibits an impressive diversity of ascomata, thallus forms and ecological requirements. The phylogenetic relationships between genera and families are poorly resolved (Baloch et al. 2010), although impressive progress has been recently achieved for the Graphidaceae (incl. Thelotremataceae), the second largest family of lichenized fungi (Rivas Plata et al. 2012). Many taxa within the subclass still require detailed phylogenetic studies. Indeed, modern statistical methods within a phylogenetical context using several loci sequences yielded interesting and quite unexpected results, such as the polyphyly of two well-known genera. Graphis is now resolved into two strongly supported clades, nested within a large clade comprizing other well-known genera such as Diorygma, Glyphis and Phaeographis (tribe Graphideae; Rivas Plata et al. 2011). Further, Pertusaria is resolved into four strongly supported groups: Pertusaria s. str. (incl. the type species Pertusaria pertusa), Pertusaria s. l. 1 including Pertusaria amara, Pertusaria s. l. 2 including Pertusaria lactea and Pertusaria velata, and a fourth group, comprizing the species with gyalectoid ascomata and recently recognized as the new genus Gyalectaria (Schmittet al. 2010).
Within such a large and very much unresolved variation, the case of the Arctomiaceae is rather simple. The family is strongly supported and includes three genera: Gregorella and Wawea, each with one species, and Arctomia with five species (Henssen 1969; Henssen and Kantvilas 1985; Jørgensen 2003, 2007; Lumbsch et al. 2005; Øvstedal and Gremmen 2001, 2006). They are lichenized with the cyanobacteria genus Nostoc, have a corticate thallus, gymnocarpous ascomata, asci with a non-amyloid thallus, and 1-10-septate, hyaline ascospores.
We here report the discovery of a further new species, which we assign to the genus Arctomia, found epiphytic in montane habitats in the island of Reunion (Mascarene archipelago, Indian Ocean). The material was first assigned to Leptogium, a genus belonging to the Collemataceae in the Lecanoromycetidae (Lumbsch and Huhndorf 2010). It is an unusual species as it has a foliose, sometimes very much crumpled, thallus, producing corticate and easily detached « goniocysts », best developed at the lobes margins, disrupting when mature and then forming a soredioid margin. Three loci were amplified (nuLSU, mtSSU, RPB1) and inferences from the sequences produced from two collections left no doubt that the material belongs to the Arctomiaceae, and statistical support to include it in the genus Arctomia was found. A new species is thus described in this genus.
Methods
Well-preserved lichen specimens lacking any visible symptoms of fungal infection were used for DNA isolation. Extraction of DNA and PCR amplification were performed following the protocol of Cubero et al. (1999). The primers used were: for nuLSU, LR0R, LR3R, LR3, LR5R and LR6 (following the suggestions available on www.lutzonilab.net/primers ), for mtSSU, mtSSU1 and mtSSU3R (Zoller et al. 1999), for RPB1, AFasc and 6R1asc (following the suggestions available on www.lutzonilab.net/primers ). Amplicons were sequenced by Macrogen®. Sequence fragments were assembled with Sequencher version 4.9 (Gene Codes Corporation, Ann Arbor, Michigan). Sequences were subjected to megaBLAST searches (Wheeler et al. 2006) to detect potential contaminations.
We assembled matrices with most representatives of species included by Lumbsch et al. (2005) in their description of the new genus Gregorella, resolved within the strongly supported Arctomiaceae; we further added several other species belonging to the Ostropomycetidae included in the study of the gyalectoid representatives of Pertusaria s.l. by Schmitt et al. (2010), assigned to the new genus Gyalectaria. All accessions available on GenBank of representatives of the Arctomiaceae were included; they represent all species assigned to that family, except for both species of Arctomia described from subantarctic islands by Øvstedal and Gremmen (2001, 2006). The outgroup species (Bacidia rosella, Lecanora intumescens and Toninia cinereovirens) were chosen outside the Ostropomycetidae and within the Lecanorales (Miadlikowska et al. 2006) to avoid any putative homoplasy problem. Six new sequences were generated for this study, all belonging to the new species described in this paper (Table 1). The sequences were first aligned using MAFFT (on-line version available at http://mafft.cbrc.jp/alignment/server/ ) and eventually manually adjusted using MacClade v. 4.05 (Maddison and Maddison 2002). Ambiguous characters have been detected by eye and excluded from the analyses.
Species and specimens used for this study, with GenBank accessions numbers for the three loci examined. Newly produced sequences for Arctomia borbonica are in bold.
Species name
LSU
mtSSU
1RPB
Absconditella sp.
AY300825
AY300873
—
Acarosporina microspora
AY584643
AY584612
DQ782818
Agyrium rufum
EF581826
EF581823
EF581822
Arctomia borbonica 1 (holotype)
JX030030
JX030032
JX030034
Arctomia borbonica 2
JX030031
JX030033
JX030035
Arctomia delicatula
AY853355
AY853307
DQ870929
Arctomia interfixa
DQ007345
DQ007348
—
Arctomia teretiuscula
DQ007346
DQ007349
DQ870930
Aspicilia contorta
DQ986782
DQ986876
DQ986852
Bacidia rosella
AY300829
AY300877
AY756412
Chromatochlamys muscorum
AY607731
AY607743
FJ941910
Coccotrema pocillarium
AF274093
AF329166
DQ870940
Conotrema populorum
AY300833
AY300882
—
Diploschistes ocellatus
HQ659183
HQ659172
DQ366252
Gregorella humida
AY853378
AY853329
—
Gyalectaria diluta
GU980982
GU980974
—
Icmadophila ericetorum
DQ883694
DQ986897
DQ883723
Lecanora intumescens
AY300841
AY300892
AY756386
Neobelonia sp.
AY300830
AY300879
—
Ochrolechia parella
AF274097
AF329173
DQ870959
Ochrolechia upsaliensis
GU980986
GU980979
GU981009
Orceolina kerguelensis
AY212830
AY212853
DQ870963
Pertusaria amara
AF274101
AY300900
DQ973048
Pertusaria lactea
AF381557
AF381564
DQ870971
Pertusaria leioplaca
AY300852
AY300903
DQ870973
Pertusaria paramerae
DQ780326
DQ780293
GU981012
Pertusaria pertusa
AF279300
AF381565
DQ870978
Pertusaria pustulata
DQ780332
DQ780297
GU981013
Pertusaria subventosa
AY300854
DQ780302
DQ870981
Placopsis gelida
AY212836
AY212859
DQ870984
Protothelenella corrosa
AY607734
AY607746
DQ870988
Protothelenella sphinctrinoidella
AY607735
AY607747
DQ870989
Thamnolia vermicularis
AY853395
AY853345
DQ915599
Thelotrema subtile
DQ871013
DQ871020
DQ870998
Toninia cinereovirens
AY756365
AY567724
AY756429
Trapelia chiodectonoides
AY212847
AY212873
DQ870999
Trapeliopsis granulosa
AF274119
AF381567
DQ871001
Wawea fruticulosa
DQ007347
DQ871023
DQ871005
Three matrices were assembled: 38 species with 927 included characters for nuLSU, 38 species with 668 included characters for mtSSU and 32 species with 675 included characters for RPB1 (part 1). Incongruence between the matrices was tested with maximum likelihood analysis using GARLI (Zwickl 2006, version 0.951 for OS X) with gaps treated as missing data, and a single most likely tree was produced. Support for the branches was estimated using bootstrap values from 1000 pseudoreplicates (all other parameters identical to the original ML search). A conflict was considered significant if a clade was supported with bootstrap support > 75% in a one-locus analysis and not in the other two. A further test for conflict was performed with LSU and RPB1 concatenated in a single matrix versus mtSSU in another. No conflict was detected and therefore the available sequences for the three loci were concatenated. The assembled matrix is deposited in TreeBASE under the accession number 12710.
An unweighted maximum parsimony (MP) analysis was performed in PAUP* 4.0b10 (Swofford 2002). All characters were equally weighted and gaps were treated as missing data. A first heuristic analysis was performed using NNI (Nearest Neighbor Interchange) branch-swapping, with 1000 replicates and saving 10 trees at each step, the functions Steepest descent and MulTrees being in effect. A second analysis was performed with the 10,000 saved trees using TBR (Tree Branch Swapping), with a maximum of 200 trees saved at each step, the function Steepest descent being inactivated. A 50% consensus tree is produced, and the strength of support for individual branches was estimated using bootstrap values (MPBS) obtained from 1000 heuristic bootstrap pseudoreplicates.
A partition of six subsets was implemented in the concatenated matrix: nuLSU, mtSSU, intron in RPB1, and three for each RPB1 codon position. Models of evolution for the maximum likelihood and Bayesian analysis were selected based on the Akaike Information Criterion (Posada and Buckley 2004) as implemented in Mr. Modeltest v2.3 (Nylander 2004). The selected model corresponds to the GTR model of nucleotide substitution (Rodríguez et al. 1990) including a proportion of invariable sites and a discrete gamma distribution of six rates categories. The maximum likelihood analysis was performed using RAxML-HPC2 (Stamatakis 2006) on the Cipres Gateway (Miller et al. 2010), with 1000 bootstrap pseudoreplicates. Bayesian analyses were carried out using the Metropolis-coupled Markov chain Monte Carlo method (MC3) in MrBayes v3.1.2 (Ronquist and Huelsenbeck 2003, Altekar et al. 2004). No priors values were assumed and gaps were treated as missing data. Four parallel runs were performed, each using four independent chains (three heated and one cold chain), with a single tree saved every 100th generation for a total of 6,000,000 generations. The incremental heating scheme was set by default. We used TRACER v1.4.1 (Rambaut and Drummond 2007) to plot the log-likelihood values of the sample points against generation time, and determine when stationarity was achieved. Consequently the first 6,000 sampled trees were deleted as the burn-in of the chain. A majority rule consensus tree with average branch lengths was constructed for the remaining trees using the sumt option of MrBayes. Phylogenetic trees were visualized using FigTree v1.3.1 (Rambaut 2009). Branches support was considered as significant when Maximum Parsimony Bootstrap (MPBS) > 70%, Maximum Likelihood Bootstrap (MLBS) > 70% and Posterior Probabilities (PP) > 0.95.
We tested the monophyly of the genus Arctomia by comparing the best unconstrained tree with the best tree obtained by constraining all Arctomia sequences to form a monophyletic group. Trees were generated in RaxML and then tested with two methods: the Shimodaira-Hasegawa (SH) test and the Expected Likelihood Weight (ELW) test as implemented in Tree-PUZZLE 5.2. (Shimodaira and Hasegawa 2001, Strimmer and Rambaut 2002, Schmidt et al. 2002).
Results
The concatenated matrix with aligned sequences for nuLSU, mtSSU and RPB1 has 2781 characters, out of which 511 are excluded (330 for nuLSU out of which 250 represent introns in Bacidia rosella, 173 for mtSSU and 8 for RPB1), 983 are constant, 276 are parsimony-uninformative and 1011 are parsimony potentially informative. The most parsimonious tree has the following characteristics: length = 6295 steps, CI = 0.336 and RI = 0.428. The ML analysis yielded a tree with a likelihood value of Ln = -28660.4 and length of 6.175. Parameters of the partitions were as follows: LSU — p(A)= 0.2604, p(C)= 0.2216, p(G)= 0.2980, p(T)= 0.2199 a= 0.3134, r(A-C)= 0.7438, r(A-G)= 1.8229, r(A-T)= 0.7430, r(C-G)= 0.7409, r(C-T)= 4.5270, r(G-T)= 1.0000; mtSSU — p(A)= 0.3330, p(C)= 0.1606, p(G)= 0.2136, p(T)= 0.2926, a= 0.4207, r(A-C)= 0.9284, r(A-G)= 2.9298, r(A-T)= 1.6160, r(C-G)= 0.6649, r(C-T)= 3.4571, r(G-T)= 1.0000; RPB1 intron — p(A)= 0.2349, p(C)= 0.2056, p(G)= 0.2267, p(T)= 0,3287, a= 0.9412, r(A-C)= 6.9358, r(A-G)= 21.9085, r(A-T)= 11.1853, r(C-G)= 8.6280, r(C-T)= 19.3378, r(G-T)= 1.0000; RPB1, 1st codon — p(A)= 0.2778, p(C)= 0.2440, p(G)= 0.3318, p(T)= 0.1463, a= 0.4211; r(A-C)= 4.0125, r(A-G)= 5.8268, r(A-T)= 3.1946, r(C-G)= 2.7176, r(C-T)= 2907386, r(G-T)= 1.0000; RPB1, 2nd codon — p(A)= 0.3521, p(C)= 0.2038, p(G)= 0.2319, p(T)= 0.2122, a= 0.3474, r(A-C)= 1.7253, r(A-G)= 3.1209, r(A-T)= 0.5159, r(C-G)= 1.9509, r(C-T)= 4.4498, r(G-T)= 1.0000; RPB1, 3rd codon — p(A)= 0.2683, p(C)= 0.2056, p(G)= 0.2545, p(T)= 0.2716, a= 0.5667, r(A-C)= 8.7546, r(A-G)= 24.9090, r(A-T)= 4.6296, r(C-G)= 5.8128, r(C-T)= 56.3087, r(G-T)= 1.0000.
All three analyses retrieve the family Arctomiaceae as a strongly supported clade (MPBS= 81%, MLBS = 97%, PP=1) (Fig. 1). All nodes within the Arctomiaceae clade are strongly supported: Arctomia delicatula and Arctomia teretiuscula form a clade supported with MLBS= 99% and PP=1.0; they further form a clade with both accessions of Arctomia borbonica that is supported with MLBS = 94% and PP=1.0; Gregorella humida and Wawea fruticulosa form a clade supported with MLBS = 86% and PP= 1.0; and finally the latter is sister to the clade including all accessions of Arctomia (except for Arctomia interfixa) in a node supported by MLBS= 95% and PP= 1.0.
50% consensus tree produced by the Bayesian analysis of a concatenated matrix with three loci (nuLSU, mtSSU and RPB1) with 2531 characters and highlighting the Arctomiaceae and the newly described Arctomia borbonica. Branches supported by MPBS and MLBS > 70% and Bayesian posterior probabilities > 0.95 are in black; those supported by MLBS >70% and Bayesian posterior probabilities > 0.95 in dark grey and those only by Bayesian posterior probabilities > 0.95 in light grey.
https://binary.pensoft.net/fig/8832
SH test shows that the likelihood of the topology constraining all Arctomia sequences to form a monophyletic group is not significantly worse (at 0.05 significance level) than that with Arctomia interfixa being sister to all other accessions of the Arctomiaceae. Following that test, the monophyly of all species assigned to Arctomia, incl. Arctomia borbonica sp. nov., cannot be rejected. The result of the ELW is the contrary: such a monophyly is rejected at 0.0473 significance level.
Discussion
The lichen family Arctomiaceae is fully recovered in our analysis (Fig. 1) and all other accessions are resolved in positions fully consistent with those published for the Ostropomycetidae (Lumbsch et al. 2005, Baloch et al. 2010, Schmitt et al. 2010), including the polyphyly of representatives of Pertusaria that are resolved in three distinct lineages, and the representative of the newly described genus Gyalectaria that is resolved as sister to the representative of Coccotrema. Our material is resolved without ambiguity within the Arctomiaceae. It is resolved with strong support as sister to a clade comprising the type species of Arctomia (Arctomia delicatula). The monophyly of the three species of Arctomia for which DNA sequences are available, demonstrated with strong support in Lumbsch et al. (2005), is not recovered in our analysis but is not rejected by the topology tests. The assignment of our new species to the genus Arctomia can thus be considered legitimate. The apparent dismemberment of Arctomia in our analysis (with Arctomia interfixa as sister to all other taxa of the Arctomiaceae) may be due to an incomplete dataset (sequences for the three loci are available for all accessions of Arctomiaceae, except for Arctomia interfixa which lacks the most informative RPB1 sequence): indeed, incomplete dataset may produce misleading results in likelihood-based analysis (Simmons 2011). However, separate analyses of LSU and mtSSU sequences yielded the same topology, with Arctomia paraphyletic. The status of Arctomia interfixa should thus be studied in more details.
Diagnostic characters for the genera recognized within the Arctomiaceae are given by Lumbsch et al. (2005). In the absence of ascomata and conidiomata, they are: thallus crustose, composed of goniocysts for Gregorella, fruticose for Wawea and crustose to coralloid or squamulose for Arctomia. The other two species of Arctomia, described by Øvstedal and Gremmen (2001, 2006) and not included in Lumbsch et al. (2005) have a thallus “placodioid” or “foliose, […] squamulose or elongate, forming rosettes”. If assigned to Arctomia, our new species does not match the thallus description of that genus, as its thallus is foliose and produces typical goniocysts at its margin, disintegrating into a soredioid margin (Fig. 2). We suggest the thallus of Arctomia borbonica is much similar to that of Wawea fruticulosa which has a “fruticose, olive-grey to brown” thallus (Henssen and Kantvilas 1985) but with lobes flattened or at least furrowed (see fig. 2 in Henssen and Kantvilas 1985, Kantvilas and Jarman 1999). Further, the structure of the cortex is quite similar in Wawea (cross section and surface view: see fig. 3A–B in Henssen and Kantvilas 1985) when compared with Arctomia borbonica (Fig. 2C–E). Finally, it is interesting to note that the sister species of Wawea is Gregorella humida whose thallus is entirely made of goniocysts, very similar to those produced by Arctomia borbonica at its thallus margin. As long as ascomata and conidiomata are not found and could provide more information, the thallus characters of Arctomia borbonica confuse the generic delimitations within the family.
The hypothesis of describing a new genus for Arctomia borbonica has been carefully assessed. Indeed, the genus as circumscribed by Henssen (1969) and Jørgensen (2007) is well-delimited and the inclusion of Arctomia borbonica makes it morphologically heterogeneous. We refrained from describing a new genus because of the following points: (a) both subantarctic species recently described by Øvstedal and Gremmen (2001, 2006) in the genus, both assumed not to genuinely belonging to Arctomia s. str. and with generic affinities “under study”, should be further studied; indeed, several characters put them aside of the genus such as a pluricellular cortex; the description of a new genus within such a small family as the Arctomiaceae is premature in that context; (b) ascomata and conidiomata are unknown, or not yet discovered, in Arctomia borbonica and thus our dataset lacks important characters (Lumbsch et al. 2005, Table 2); (c) morphological and anatomical characters may be very much misleading for phylogenetic reconstruction and sound generic delimitations as demonstrated by many studies in lichenized or unlichenized ascomycetes (Gaya et al. 2008, Lantz et al. 2011, Prieto et al. 2012, Sérusiaux et al. 2010); and (d) two statistical topology tests applied to the likelihood tree gave opposite results to assess the monophyly of Arctomia when including all species studied, e.g. Arctomia borbonica, Arctomia delicatula, Arctomia interfixa and Arctomia teretiuscula.
Species recognized by its foliose, usually much crumpled, blue grey to brown thallus producing goniocysts at its margins, eventually forming a soredioid margin. Ascomata and conidiomata unknown.
Type.
REUNION (Mascarene archipelago). Forêt de Bébour, track starting at Gîte de Bélouve toward Piton des Neiges, 21°4'49"S, 55°31'24"E (DMS), 1850 m alt., 9 Nov 2009, wet montane ericoid tickets, N. Magain & E. Sérusiaux sn (holotype : LG).
Description.
Thallus not exceeding 1 cm in diam., with distinct lobes when well-developed, lobes blue-grey to brown when dry, up to 0.2-0.3 mm wide and c. 200-400 µm thick, hardly distinguished in some specimens, with a surface typically wrinkled (even in young lobes), sometimes very much “crumpled”, always developing small goniocysts, mainly at the margins but also on the upper surface; cortex (Fig. 2C–E) developed on upper and lower sides, formed by a single layer of small rounded (in cross section) and jigsaw-like (in surface view) cells, less than 5 µm thick; goniocysts (Fig. 2F) 20-80 µm across, always containing compact chains of Nostoc cells and covered by a layer of isodiametric to rounded cells, 2–5 µm, best developed at the lobes margins where they eventually form a typical pale brownish soredioid edge, due to cortical disintegration. Photobiont belonging to the cyanobacteria genus Nostoc forming chains of small rounded cells 2–5 µm in diam. Ascomata and conidiomata unknown.
Arctomia borbonica (holotype). A–B macroscopic view of the thallus, with details of the wrinkled surface B and soredioid margin, made of disintegrating goniocysts C–D cross section through the thallus, showing the cortex with small, isodiametric cells, and the Nostoc chains E surface view of the cortex F young goniocysts formed at the lobes margins. Scale: A–B = 1 mm; C–E = 20 µm.
https://binary.pensoft.net/fig/8833Chemistry.
No secondary metabolites found by TLC.
Notes.
The material looks like a species in Leptogium, a genus belonging to the Collemataceae in the Lecanoromycetidae (Lumbsch and Huhndorf 2010). Soredia or soredioid propagules are however unknown in that genus as well as in the closely related Collema. Arctomia borbonica is easily recognized by its foliose, sometimes very much crumpled, blue grey to brown thallus, producing corticate and easily detached « goniocysts », best developed at the lobes margins, disrupting when mature and then forming a soredioid margin.
Distribution and ecology.
Arctomia borbonica has been collected at three different sites on the island of Reunion in the Mascarene archipepago, incl. in highly disturbed secondary tickets with Eucalyptus plantations; it grows on trunks (Eucalyptus, Acacia heterophylla) or on main stems of Erica tickets. It is probably widespread on the island. The two localities with natural vegetation belong to two different and typical habitats. The first one is the margin of the “Forêt de tamarins des hauts” with large boles of the endemic tree Acacia heterophylla (locality at the nature reserve “Roche Ecrite”, at 1500 m) and corresponds to the “Acacia mountain forest” in Strasberg et al. (2005). The other one is the wet upper montane ericoid tickets (type locality; locality in the Bébour forest at 1800–1850 m) and corresponds to the “Philippia mountain ticket” in Strasberg et al. (2005). Here the vegetation does not exceed 4–5 m in height and is formed by Erica arborescens, Erica montana, Eugenia buxifolia, Agauria buxifolia, Cordyline mauritiana (locally very abundant), Cyathea sp., Phylica nitida, Astelia hemichrysa, Blechnum attenuatum; ground is covered by very thick (up to 80 cm) layer of Sphagnum and other bryophytes. It is one of the most rewarding habitat for lichens on Reunion, with many interesting species, including representatives of the austral element (van den Boom et al. 2011), such as Gomphillus morchelloides, Gomphillus pedersenii and Sporopodiopsis mortimeriana.
Other specimens examined.
REUNION (Masarenes archipelago). Nature reserve at Roche Ecrite, track to the summit, 20°58'6"S, 55°26'26"E (DMS), c. 1500 m alt., 4 nov 2009, montane forest dominated by Acacia heterophylla, N. Magain & E. Sérusiaux sn (LG). S part of the island, N of St-Philippe, near « gîte Bernard Brice », 21°20'23"S, 55°41'55"E (DMS), 650 m alt., 10 Nov 2009, Eucalyptus plantations and secondary tickets, N. Magain & E. Sérusiaux sn (LG).
Acknowledgements
Field studies in Reunion were made possible with the help and advice from the Parc National de La Réunion, especially through the courtesy of Mr B. Lequette and Mr J. M. Pausé. Dr Cl. Ah-Peng and Prof. D. Strasberg of the University of La Réunion in Saint-Denis and Dr. J. Hivert of the Conservatoire Botanique National de Mascarin (St-Leu) were also very helpful. We thank them all most sincerely. We further thank Mr I. Cremasco and L. Gohy for technical assistance in the molecular laboratory and herbarium at the University of Liège. Nicolas Magain is a Ph.D. Student at the University of Liège and acknowledges the financial support by FRIA, an organ of the Belgian Research Foundation. Finally we thank both referees for their critical and helpful notes and suggestions.
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